Molecular characterization of bovine placental and ovarian 20α-hydroxysteroid dehydrogenase

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Molecular characterization of bovine placental and ovarian 20α-hydroxysteroid dehydrogenase

The enzyme 20α-hydroxysteroid dehydrogenase (20α-HSD) catalyzes the conversion of progesterone to its inactive form, 20α-hydroxyprogesterone. This enzyme plays a critical role in the regulation of luteal function in female mammals. In this study, we conducted the characterization and functional analyses of bovine 20α-HSD from placental and ovarian tissues. The nucleotide sequence of bovine 20α-...

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Changes in Ovarian and Placental 20α-hydroxysteroid Dehydrogenase Activity during the Pregnancy in the Rat

The enzyme 20α-hydroxysteroid dehydrogenase (20α-HSD) catabolizes progesterone to 20α-dihydroprogesterone (20α-OHP), and is appeared in rat corpora luteal and placenta. A polled samples of 10-15 placental and ovarian tissues collected from each individual rat were subjected to measurement of 20α-HSD activity. A 20α-HSD activity in the cytosol fraction was based on the generation of NADPH. In th...

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Dehydrogenase and oxoreductase activities of porcine placental 11beta-hydroxysteroid dehydrogenase.

Dehydrogenase (cortisol to cortisone) and oxoreductase (cortisone to cortisol) activities of porcine placental 11beta-hydroxysteroid dehydrogenase (11beta-HSD) were measured in tissue fragment cultures on day 75 of gestation. Dehydrogenase activity was over fivefold greater than oxoreductase activity (p < .001). There were positive linear associations (p < .01) between net dehydrogenase activit...

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Placental 11 -Hydroxysteroid Dehydrogenase in Dahl and Spontaneously Hypertensive Rats

Studies in normotensive rats showed that excessive fetal exposure to maternal glucocorticoids retards growth and programs hypertension in later life. This excessive exposure is proposed to occur due to a reduction of the placental barrier to maternal glucocorticoids that is provided by 11 -hydroxysteroid dehydrogenase (11 HSD). To assess the possible alterations of glucocorticoid placental barr...

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Characterization of a human 20 -hydroxysteroid dehydrogenase

It has been suggested that 20 -hydroxysteroid dehydrogenase (20 -HSD) is a T-cell differentiation marker in mice. In the human, this enzyme has generally been associated with types 1 and 2 17 -HSDs, which belong to the short-chain alcohol dehydrogenase family, whereas the rat, rabbit, pig and bovine 20 -HSDs are members of the aldoketo reductase superfamily, which also includes the 3 -HSD famil...

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ژورنال

عنوان ژورنال: REPRODUCTION

سال: 2011

ISSN: 1470-1626,1741-7899

DOI: 10.1530/rep-11-0093